일반대학원공과대학분자과학기술학과

List

이재성Jae Seong Lee

  • 소속 분자과학기술학과
  • 연구실혜강관 422호
  • 이메일 jaeseonglee@ajou.ac.kr
  • 내선번호0312193896

관심분야

  • 치료용 단백질 생산, 동물세포공학, 동물세포 시스템 및 합성생물학

학력

  • 2012.02 한국과학기술원 박사
  • 2007.02 한국과학기술원 학사

경력

    2021 - Present     Associate Professor, Ajou University
2017 - 2021        Assistant Professor, Ajou University
2016 - 2017        Research Scientist, Danmarks Tekniske Universitet (DTU), Denmark
2013 - 2015        Post-doctoral Fellow, Danmarks Tekniske Universitet (DTU), Denmark
2012 - 2013        Post-doctoral Fellow, KAIST, Korea

연구분야

    Mammalian cell culture for therapeutic protein production
Mammalian cell line engineering - Genome engineering/Metabolic engineering
Genome editing technologies
Tool development for mammalian synthetic biology 
Omics technologies - Genomics/Transcriptomics/Proteomics

대표논문

  • [논문] 신승현, 김수현, 이재성, 이균민, Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins, ACS SYNTHETIC BIOLOGY, pp. 1-13 (6월, 2021)
  • [논문] 김동우, 신성욱, 이재성, Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22, No.5, pp. 2407-2419 (2월, 2021)
  • [논문] 신승현, 신성욱, Lasse Ebdrup Pedersen, Lise Marie Grav, 김수현, 이재성, 이균민, Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells, ACS SYNTHETIC BIOLOGY, Vol.9, No.6, pp. 1263-1269 (5월, 2020)
  • [논문] 신성욱, 이재성, Optimized CRISPR/Cas9 strategy for homology‐directed multiple targeted integration of transgenes in CHO cells, BIOTECHNOLOGY AND BIOENGINEERING, pp. 1-9 (2월, 2020)
  • [논문] Helene Faustrup Kildegaard, Nathan E. Lewis, 이재성, 이균민, Mitigating Clonal Variation in Recombinant Mammalian Cell Lines, TRENDS IN BIOTECHNOLOGY, pp. 931-942 (9월, 2019)

연구활동

  • [논문] Su Hyun Kim, 신성욱, Dahyun Chun, Jiseon Yoo, Jong-Ho Park, Seunghyeon Shin, Weon-Kyoo You, Yeon-Gu Kim, 이재성, Gyun Min Lee, Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP−SETDB1 Complex for Enhancing Difficult-to-Express Protein Production, ACS Synthetic Biology, Vol.13, pp. 634-647 (1월, 2024)
  • [논문] 김동우, 김슬미, 김지원, 이재진, 이재성, Knockout of the lysosomal membrane protein, LAMP2C, improves transient gene expression in HEK293 cells via increased intracellular plasmid availability, Biotechnology Journal, Vol.19, No.1, pp. 2300017-2300028 (1월, 2024)
  • [논문] 김수현, Helene Faustrup Kildegaard, Hooman Hefzi, Kai Xiong, Karen Julie la Cour Karottki , Lasse Ebdrup Pedersen, Lise Marie Grav, Nathan E. Lewis, 백민혜, 신승현, 이재성, 이균민, Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening, METABOLIC ENGINEERING, pp. 66-77 (11월, 2023)
  • [논문] 김동우, 이재성, Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells, BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, pp. 289-299 (3월, 2023)
  • [논문] 김슬미, 이재진, 이재성, Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9-mediated knock-in in mammalian cells, ENGINEERING IN LIFE SCIENCES, pp. 1-9 (3월, 2023)
  • [논문] 민홍기, 김동우, 김슬미, 이솔휘, 이수민, 이재성, Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells, FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, pp. 977193-977206 (9월, 2022)
  • [논문] 신승현, 김수현, 박종호, 이재성, 이균민, Recombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress, METABOLIC ENGINEERING, Vol.72, pp. 247-258 (7월, 2022)
  • [논문] 경민지, 이재성, Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells, METABOLIC ENGINEERING, Vol.72, pp. 35-45 (7월, 2022)
  • [논문] 김수현, 박성제, 백민혜, 신승현, 이재성, 이균민, Improving the secretory capacity of CHO producer cells: The effect of controlled Blimp1 expression, a master transcription factor for plasma cells, METABOLIC ENGINEERING, Vol.69, pp. 73-86 (1월, 2022)
  • [논문] 민홍기, 장민호, 이재성, Enhancement of Transgene Expression by Mild Hypothermia Is Promoter Dependent in HEK293 Cells, LIFE, Vol.11, No.9, pp. 901-908 (8월, 2021)
  • [논문] Kai Xiong, 이재성, Helene Faustrup Kildegaard, Hooman Hefzi, Ildze Ventina, Karen Julie la Cour Karottki, Lise Marie Grav, Nathan E. Lewis, Philipp Spahn, Shangzhong Li, Songyuan Li, 이균민, Lasse Ebdrup Pedersen, An optimized genome-wide, virus-free CRISPR screen for mammalian cells, CELL REPORTS METHODS, Vol.1, No.4, pp. 100062-100072 (8월, 2021)
  • [논문] 신승현, 김수현, 이재성, 이균민, Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins, ACS SYNTHETIC BIOLOGY, pp. 1-13 (6월, 2021)
  • [논문] Karen Julie la Cour Karottki, 이재성, Alex Thomas, Chintan Joshi, David Ruckerbauer, Hooman Hefzi, Juan A.Hernandez Bort, Lasse Ebdrup Pedersen, Nicole Borth, Philipp N. Spahn, Songyuan Li, 이균민, Helene Faustrup Kildegaard, Nathan E. Lewis, A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes, METABOLIC ENGINEERING, Vol.66, pp. 114-122 (4월, 2021)
  • [논문] 김동우, 신성욱, 이재성, Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22, No.5, pp. 2407-2419 (2월, 2021)
  • [논문] 곽진명, 신성욱, 이영식, 이재성, Hydroxyurea selection for enhancement of homology-directed targeted integration of transgenes in CHO cells, NEW BIOTECHNOLOGY, Vol.62, pp. 26-31 (1월, 2021)
  • [논문] 신성욱, 이재성, CHO Cell Line Development and Engineering via Site-specific Integration: Challenges and Opportunities, BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, Vol.25, pp. 633-645 (10월, 2020)
  • [논문] 이영식, 곽진명, 이재성, Endogenous p21-Dependent Transgene Control for CHO Cell Engineering, ACS SYNTHETIC BIOLOGY, Vol.9, No.7, pp. 1572-1580 (6월, 2020)
  • [논문] 신승현, 신성욱, Lasse Ebdrup Pedersen, Lise Marie Grav, 김수현, 이재성, 이균민, Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells, ACS SYNTHETIC BIOLOGY, Vol.9, No.6, pp. 1263-1269 (5월, 2020)
  • [논문] 신성욱, 이재성, Optimized CRISPR/Cas9 strategy for homology‐directed multiple targeted integration of transgenes in CHO cells, BIOTECHNOLOGY AND BIOENGINEERING, pp. 1-9 (2월, 2020)
  • [논문] Karen Julie la Cour Karottki, 이재성, Anders Holmgaard Hansen, Hooman Hefzi, Isaac Shamie, Kai Xiong, Lasse Ebdrup Pedersen, Shangzhong Li, Songyuan Li, 이균민, Helene Faustrup Kildegaard, Nathan E. Lewis, Awakening dormant glycosyltransferases in CHO cells with CRISPRa, BIOTECHNOLOGY AND BIOENGINEERING, pp. 1-6 (10월, 2019)
  • [논문] Helene Faustrup Kildegaard, Nathan E. Lewis, 이재성, 이균민, Mitigating Clonal Variation in Recombinant Mammalian Cell Lines, TRENDS IN BIOTECHNOLOGY, pp. 931-942 (9월, 2019)
  • [논문] Daria Sergeeva, 이재성, Jose Manuel Camacho-Zaragoza, Helene Faustrup Kildegaard, CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells, Methods in molecular biology (Clifton, N.J.), Vol.1961, pp. 213-232 (3월, 2019)
  • [논문] Kai Xiong, Karen Julie la Cour Karottki, Kim Fabiano Marquart, 이재성, Alejandro Chavez, Isaac Shamie, Nan Cher Yeo, Shangzhong Li, Signe Gerling, 이균민, Helene Faustrup Kildegaard, Nathan E. Lewis, Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference., BIOTECHNOLOGY AND BIOENGINEERING, Vol.116, No.7, pp. 1813-1819 (3월, 2019)
  • [논문] Bernhard O. Palsson, Helene Faustrup Kildegaard, Mojtaba Samoudi, Nathan E. Lewis, 박진형, 하태광, 이재성, 이균민, Revealing Key Determinants of Clonal Variation in Transgene Expression in Recombinant CHO Cells Using Targeted Genome Editing, ACS SYNTHETIC BIOLOGY, pp. A-L (11월, 2018)
  • [논문] Søren D. Petersen, 이재성, Helene F. Kildegaard, Jay D. Keasling, Jie Zhang, Lise M. Grav, Tadas Jakoˇ ci ?unas1, Michael K. Jensen, Modular 5'-UTR hexamers for context-independent tuning of protein expression in eukaryotes., NUCLEIC ACIDS RESEARCH, pp. NA-NA (8월, 2018)
  • [논문] Lise Marie Grav, 이재성, Daria Sergeeva, Igor Marin de Mas, Lars Keld Nielsen, Mikael Rørdam Andersen, Nathan E. Lewis, 이균민, Helene Faustrup Kildegaard, Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation., ACS SYNTHETIC BIOLOGY, pp. NA-NA (7월, 2018)
  • [논문] Eric Baek, 이재성, 이균민, Untangling the mechanism of 3-methyladenine in enhancing the specific productivity: Transcriptome analysis of recombinant Chinese hamster ovary cells treated with 3-methyladenine., BIOTECHNOLOGY AND BIOENGINEERING, Vol.115, No.9, pp. 2243-2254 (6월, 2018)
  • [논문] Lise Marie Grav, 이재성, Karen Julie la Cour Karottki, Helene Faustrup Kildegaard, Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells, Methods in molecular biology (Clifton, N.J.), pp. 101-118 (5월, 2017)
  • [논문] Hooman Hefzi, Kok Siong Ang, Michael Hanscho, 이재성, Aarash Bordbar, Alex Thomas, Alyaa M.Abdel-Haleem, Anne Mathilde Lund, Bernhard O.Palsson, Bjørn G.Voldborg, Camila A.Orellana, Daniel C.Zielinski, Daniel Ley, David Ruckerbauer, Deniz Baycin-Hizal, Giuseppe Paglia, Harish Nagarajan, Helene F.Kildegaard, Jahir M.Gutierrez, Johnny Arnsdorf, Juergen Zanghellini, Lake-Ee Quek, Lars K.Nielsen, Lasse E.Pedersen, Meiyappan Lakshmanan, Michael J.Betenbaugh, Mikael R.Andersen, Natalia E.Jim?nez, Nicolas Loira, Philipp N.Spahn, Sarantos Kyriakopoulos, Shangzhong Li, Tune Wulff, Veronica S.Martinez, Yingxiang Huang, Zachary A.King, Ziomara P.Gerdtzen, Dong-Yup Lee, Nathan E.Lewis, Nicole Borth, A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism, CELL SYSTEMS, Vol.3, No.5, pp. 434-443 (11월, 2016)
  • [논문] 이재성, Lasse Ebdrup Pedersen, Lise Marie Grav, 이균민, Helene Faustrup Kildegaard, Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment, BIOTECHNOLOGY AND BIOENGINEERING, Vol.113, No.11, pp. 2518-2523 (6월, 2016)
  • [논문] Eric Baek, 김채린, 이재성, 김미겸, 이균민, Chemical inhibition of autophagy: Examining its potential to increase the specific productivity of recombinant CHO cell lines, BIOTECHNOLOGY AND BIOENGINEERING, pp. 1953-1961 (3월, 2016)
  • [논문] 이재성, Lise Marie Grav, Anders Holmgaard Hansen, Lasse Ebdrup Pedersen, Signe Gerling, Stefan Kol, Thomas Beuchert Kallehauge, 이균민, Helene Faustrup Kildegaard, One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment, BIOTECHNOLOGY JOURNAL, Vol.10, No.9, pp. 1446-1456 (9월, 2015)
  • [논문] 이재성, Lise Marie Grav, Nathan E. Lewis, Helene Faustrup Kildegaard, CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives, BIOTECHNOLOGY JOURNAL, Vol.10, pp. 979-994 (7월, 2015)
  • [논문] 이재성, Lasse Ebdrup Pedersen, Thomas Beuchert Kallehauge, Helene Faustrup Kildegaard, Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway, SCIENTIFIC REPORTS, Vol.5, pp. 8572-8572 (2월, 2015)
해당 데이터는 존재하지 않습니다.
  • [학술회의] 이재성, Genome-Wide Virus-Free CRISPR Screening Platform for Identifying Novel Engineering Targets in Mammalian Cells, 15th Annual PEGS Europe, (11월, 2023)
  • [학술회의] 신성욱, 이재성, 김지원, 민홍기, Quantitative Gene Expression Control Using the ROSE LP Platform: A Reproducible and Sustainable Transcription Regulation for CHO Cell Engineering, The 16th Conference on Protein Expression in Animal Cells, (9월, 2023)
  • [학술회의] 이재성, Engineering Mammalian Cell Lines to Overcome Production of Difficult-to-Express Proteins, 2023 Antibody Society Korea Summer Conference, (7월, 2023)
  • [학술회의] 이재성, Emerging Mammalian Cell Engineering Strategy for Production of Difficult-to-Express Proteins, KMB 2023 Annual Meeting & International Symposium, (6월, 2023)
  • [학술회의] 경민지, 이재성, Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells, 27th ESACT Meeting (European Society for Animal Cell Technology), (6월, 2022)
  • [학술회의] 이재성, 신성욱, An Inducible Platform CHO Cell Line for Stoichiometric Functional Gene Study by Doxycycline-inducible and Recombinase-mediated Cassette Exchange System, 2022 KSBB Spring Meeting and International Symposium, (4월, 2022)
  • [학술회의] 민홍기, 이재성, 김동우, 김슬미, 이솔휘, 이수민, Hybrid Cell Line Development System Utilizing Site-specific Integration and Methotrexate-mediated Gene Amplification in Chinese Hamster Ovary Cells, 2022 KSBB Spring Meeting and International Symposium, (4월, 2022)
  • [학술회의] 이재성, 이균민, Bernhard O. Palsson, Helene Faustrup Kildegaard, Mojtaba Samoudi, Nathan E. Lewis, 박진형, 하태광, Genomic Understanding of Variation in Transgene Expression in CHO, 26th Meeting of the European Society for Animal Cell Technology, (5월, 2019)
  • [학술회의] 이재성, Toward synthetic biology-driven mammalian cell line development for therapeutic protein production, 2023 한국공업화학회 추계 총회 및 학술대회, (5월, 2023)
  • [학술회의] 김슬미, 이재성, Artificial Host Spot Included UCOE to Enable Efficient Knock-in and High and Stable Transgene Expression in Mammalian Cells, 2021 KSBB Spring Meeting and International Symposium, (4월, 2021)
  • [학술회의] 경민지, 이재성, Unfolded Protein Response Monitoring System for Intuitive Identification of ER Stress in CHO Cells, 2021 KSBB Spring Meeting and International Symposium, (4월, 2021)
  • [학술회의] 경민지, 이재성, ER Stress-driven BCL-2 Overexpression Inhibits Cell Death in CHO Cells, 2020 KSBB Fall Meeting and International Symposium: Hybrid Conference, (10월, 2020)
  • [학술회의] 김동우, 신성욱, 이재성, Vector Construct Optimization of CRISPR/Cas9 Components for Efficient Multiple Site-specific Integration in CHO cells, 2020 KSBB Fall Meeting and International Symposium: Hybrid Conference, (10월, 2020)
  • [학술회의] 김동우, 이재성, Cas9-Zinc Finger System for Efficient HDR-mediated Knock-In in CHO Cells, 2020 KSBB Fall Meeting and International Symposium: Hybrid Conference, (10월, 2020)
  • [학술회의] 이재성, Advancing Cell Line Development in the Era of Genome Editing, 2019 KSBB Fall Meeting and International Symposium, (10월, 2019)
  • [학술회의] 신성욱, 이재성, An Optimized Strategy for Efficient Multiple Targeted Integration with CRISPR/Cas9-mediated Homology-Directed Repair Pathway in CHO cells, 2019 KSBB Fall Meeting and International Symposium, (10월, 2019)
  • [학술회의] 이재성, Next Generation Cell Line Development Platform for Therapeutic Protein Production, KMB 2018 Annual Meeting & International Symposium, (6월, 2018)
  • [학술회의] 이재성, Designing mammalian cells for production of tailor-made biopharmaceuticals, 2017 한국공업화학회 추계 총회 및 학술대회, (11월, 2017)
  • [학술회의] 이재성, Designing Mammalian Cells toward Controlled and Efficient Production of Therapeutic Proteins, 2017 KSBB Fall Meeting and International Symposium, (10월, 2017)
  • [저서] 이재성, 경민지, 신성욱, [Book Chapter] Next-Generation Cell Engineering Platform for Improving Recombinant Protein Production in Mammalian Cells, pp. 189~224- (1월, 2022)
  • [저서] 이재성, 이균민, 하태광, Cell Culture Engineering: Recombinant Protein Production, Chapter 1. Platform Technology for Therapeutic Protein Production, pp. 1-22- (9월, 2019)
  • [저서] Daria Sergeeva, Helene Faustrup Kildegaard, 이재성, Karen Julie la Cour Karottki, Cell Culture Engineering: Recombinant Protein Production, Chapter 8. CRISPR Toolbox for Mammalian Cell Engineering, pp. 185-206- (9월, 2019)

특허 및 기타

  • [특허] 이재성, 김동우, 김슬미, LAMP2C 유전자가 녹아웃 된 형질전환 인간 세포주 및 이를 이용한 목적 유전자의 생산 방법, 특허, (등록) (10-2576635) (9월, 2023)
  • [특허] 이재성, 이영식, 히드록시우레아를 유효성분으로 함유하는 세포주기 조절방법 및 위치특이적 유전자 삽입 효율 증가 방법, 특허, (출원) (10-2020-0034554) (3월, 2020)