<분자과학기술세미나> 안내 드립니다. 

♠ 주제 : A Biphasic Osteovascular Biomimetic Scaffold for Rapid and Self‐Sustained Endochondral Ossification

♠ 연사 : 김환 교수님 (한국교통대학교)         

♠ 일시 : 2021년 9월 28일 17:00~18:00  

♠ 초청 : 김재호교수님(내선 2517) 

Abstract 

Hwan D. Kim, Department of Polymer Science and Engineering, Korea National University of Transporation

Regeneration of large bones remains a challenge in surgery. Recent developmental engineering efforts aim to recapitulate endochondral ossification (EO), a critical step in bone formation. However, this process entails the condensation of mesenchymal stem cells (MSCs) into cartilaginous templates, which requires long-term cultures and is challenging to scale up. Here, a biomimetic scaffold is developed that allows rapid and self-sustained EO without initial hypertrophic chondrogenesis. The design comprises a porous chondroitin sulfate cryogel decorated with whitlockite calcium phosphate nanoparticles, and a soft hydrogel occupying the porous space. This composite scaffold enables human endothelial colony-forming cells (ECFCs) and MSCs to rapidly assemble into osteovascular niches in immunodeficient mice. These niches contain ECFC-lined blood vessels and perivascular MSCs that differentiate into RUNX2+OSX+ pre-osteoblasts after one week in vivo. Subsequently, multiple ossification centers are formed, leading to de novo bone tissue formation by eight weeks, including mature human OCN+OPN+ osteoblasts, collagen-rich mineralized extracellular matrix, hydroxyapatite, osteoclast activity, and gradual mechanical competence. The early establishment of blood vessels is essential, and grafts that do not contain ECFCs fail to produce osteovascular niches and ossification centers. The findings suggest a novel bioengineering approach to recapitulate EO in the context of human bone regeneration.

♠ ZOOM LINK 

Topic: MST6021_ 분자과학기술세미나Ⅳ(O1423-1) [김환 교수님]

Time: Sep 28, 2021 05:00 PM Seoul

https://ajou-ac-kr.zoom.us/j/81199748246?pwd=MzI0RUVoT0d0VVJHeDcxWGJkR1RvUT09

Meeting ID: 811 9974 8246

Passcode: 778859