♠ 주제 : Lectin nanoparticles in a dielectrophoretic microchip for the specific capture of exosomes secreted from pancreatic cancer cells

♠ 연사 : 최종훈 교수님 (중앙대학교 융합공학부)        

♠ 일시 : 2021년 10월 19일 17:00~18:00  

♠ 초청 : 유태현교수님(내선 3543)  

Abstract

The unique profile of upregulated glycosylation in metastatic cancer cells may form the basis for the development of new biomarkers for the targeting and diagnosis of specific cancers. This study introduces a pancreatic cancer cell-derived exosome detection technology, which is based on the specific binding of lectins to distinctive glycan profiles on the surface of exosomes. Lectins with a high and specific affinity for sialic acid or fucose were attached to bifunctional Janus nanoparticles (JNPs), which facilitated interactions with pancreatic cancer cell-derived exosomes in a microfluidic device. Here, we show that pancreatic cancer cell-derived exosomes from two cell lines and plasma samples collected from patients diagnosed with pancreatic cancer were successfully captured on the lectin-conjugated JNPs with affinities that were comparable to those of CA19-9, a conventional antibody. In addition, exosome detection using our platform could differentiate between metastatic and nonmetastatic pancreatic cancer cells. This study opens the possibility to achieve a new early diagnosis marker based on the glycan properties of pancreatic cancer cell-derived exosomes. 

♠줌링크

Topic: MST6016_ 분자과학기술세미나Ⅱ(O1413-1) [최종훈교수님]

Time: Oct 19, 2021 05:00 PM Seoul

https://ajou-ac-kr.zoom.us/j/88165259734?pwd=ZDQ0V3JYZERBRVNWSXREelIvMXFPdz09

Meeting ID: 881 6525 9734

Passcode: 624378